Evaluation of PCR assays for quantifying seed-borne infection by Fusarium and Microdochium seedling blight pathogens
نویسندگان
چکیده
منابع مشابه
Improved template preparation for PCR-based assays for detection of food-borne bacterial pathogens.
Shigella flexneri, Salmonella enterica serotype Typhimurium, and Listeria monocytogenes were applied to FTA filters, and the filters were used directly as templates to demonstrate their sensitivity and applicability in PCR-based detection assays. With pure cultures, the sensitivities of detection by FTA filter-based PCR were 30 to 50 and 200 CFU for the gram-negative enterics and Listeria, resp...
متن کاملNovel Fusarium head blight pathogens from Nepal and Louisiana revealed by multilocus genealogical concordance.
This study was conducted to assess evolutionary relationships, species diversity and trichothecene toxin potential of five Fusarium graminearum complex (FGSC) isolates identified as genetically novel during prior Fusarium head blight (FHB) surveys in Nepal and Louisiana. Results of a multilocus genotyping (MLGT) assay for B-trichothecene species determination indicated these isolates might repr...
متن کاملResistance to Fusarium head blight and seedling blight in wheat is associated with activation of a cytochrome p450 gene.
ABSTRACT One plant genotype displays a resistance phenotype at one development stage but a susceptible reaction to the same pathogen at another stage, which is referred to here as resistance inversion. In wheat, Fusarium head blight (FHB)-resistant cv. Sumai3 showed a Fusarium seedling blight (FSB)-susceptible reaction whereas FHB-susceptible cv. Annong8455 exhibited FSB resistance when challen...
متن کاملexamining the relationship between fear of negative evaluation and communication strategies by iranian efl learners
the purpose of the present study was to investigate the relationship between fear of negative evaluation (fne) and communication strategies (css) among iranian efl learners. it was aimed to examine the differences in the use of communication strategies between speakers with high or low degree of fear of negative evaluation. the current study was a case study consisting of 10 english learners at...
Fusarium Head Blight of Wheat
Nebraska–Lincoln cooperating with the Counties and the United States Department of Agriculture. University of Nebraska–Lincoln Extension educational programs abide with the nondiscrimination policies of the University of Nebraska–Lincoln and the United States Department of Agriculture. © 2008, The Board of Regents of the University of Nebraska on behalf of the University of Nebraska–Lincoln Ext...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2010
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2009.04410.x